endometrial cancer cell lines ishikawa  (Procell Inc)

Journal: International Journal of Molecular Sciences

Article Title: Differential Effects of Sphingolipids on Cell Death and Antioxidant Defenses in Type 1 and Type 2 Endometrial Cancer Cells

doi: 10.3390/ijms26104472

Figure Lengend Snippet: ( A ) The impact of Sptlc1 gene silencing on Sptlc1 mRNA in Ishikawa and HEC-1A cells; ( B ) The impact of Sptlc2 gene silencing on Sptlc2 mRNA in Ishikawa and HEC-1A cells; ( C ) The impact of Sptlc1 gene silencing on SPT1 protein content in Ishikawa and HEC-1A cells; ( D ) The impact of Sptlc2 gene silencing on SPT2 protein content in Ishikawa and HEC-1A cells; Data are presented as medians (interquartile range). The difference between groups and compared by Mann–Whitney U test: ** p < 0.01, **** p < 0.0001 vs. control group; b p < 0.01, vs. Ishikawa (CON) group.

Article Snippet: The human endometrial cancer cell line Ishikawa (representing type 1 endometrial cancer) was purchased from Merck, while HEC-1-A (representing type 2 endometrial cancer) was obtained from the American Type Culture Collection (ATCC).

Techniques: MANN-WHITNEY, Control

Journal: International Journal of Molecular Sciences

Article Title: Differential Effects of Sphingolipids on Cell Death and Antioxidant Defenses in Type 1 and Type 2 Endometrial Cancer Cells

doi: 10.3390/ijms26104472

Figure Lengend Snippet: ( A ) The impact of Sptlc1 and Sptlc2 gene silencing on cell viability in Ishikawa and HEC-1A cells; ( B ) The impact of Sptlc1 and Sptlc2 gene silencing on cell death in Ishikawa and HEC-1A cells. Data are presented as medians (interquartile range). The difference between groups and compared by Mann–Whitney U test: *** p < 0.001, **** p < 0.0001 vs. control group.

Article Snippet: The human endometrial cancer cell line Ishikawa (representing type 1 endometrial cancer) was purchased from Merck, while HEC-1-A (representing type 2 endometrial cancer) was obtained from the American Type Culture Collection (ATCC).

Techniques: MANN-WHITNEY, Control

Journal: International Journal of Molecular Sciences

Article Title: Differential Effects of Sphingolipids on Cell Death and Antioxidant Defenses in Type 1 and Type 2 Endometrial Cancer Cells

doi: 10.3390/ijms26104472

Figure Lengend Snippet: ( A ) The impact of Sptlc1 and Sptlc2 gene silencing on ROS levels in Ishikawa and HEC-1A cells; ( B ) The impact of Sptlc1 and Sptlc2 gene silencing on catalase activity in Ishikawa and HEC-1A cells; ( C ) The impact of Sptlc1 and Sptlc2 gene silencing on TAC in Ishikawa and HEC-1A cells; ( D ) The impact of Sptlc1 and Sptlc2 gene silencing on caspase-3 activity in Ishikawa and HEC-1A cells; Data are presented as medians (interquartile range). The difference between groups and compared by Mann–Whitney U test: ** p < 0.01, *** p <0.001, **** p < 0.0001 vs. control group; b p < 0.01, d p < 0.0001 vs. Ishikawa (CON) group.

Article Snippet: The human endometrial cancer cell line Ishikawa (representing type 1 endometrial cancer) was purchased from Merck, while HEC-1-A (representing type 2 endometrial cancer) was obtained from the American Type Culture Collection (ATCC).

Techniques: Activity Assay, MANN-WHITNEY, Control

Journal: Molecular Medicine

Article Title: Celastrol promotes DNA damage and apoptosis in uterine corpus endometrial carcinoma via promotion of KAT2B-mediated RBPJ acetylation and repression of MCM4 transcription

doi: 10.1186/s10020-025-01082-z

Figure Lengend Snippet: Celastrol suppresses cell proliferation in UCEC. Four UCEC cell lines Ishikawa, HEC-1-B, KLE and HEC-1-A were applied and treated with different concentrations of Celastrol (0, 0.3, 0.625, 1.25, 2.5, 5, 10 or 20 µM) for 24 h. ( A ) The chemical structure of Celastrol. ( B ) CCK8 assay tested the cell viability, and cell inhibition rate (CI) was calculated. ( C ) Ishikawa and HEC-1-A cells were treated with 2.5, 5 or 10 µM of Celastrol (L-Celastrol, M-Celastrol or H-Celastrol) and cultured for 24 h. The distribution of cell cycle was evaluated by flow cytometry analysis. ( D ) Cell cycle-associated protein expression levels were measured by western blotting assays. All the values are mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01 vs. the control group

Article Snippet: The human endometrial cancer cell lines Ishikawa, HEC-1-B, KLE and HEC-1-A were obtained from iCell Bioscience Inc (Shanghai, China).

Techniques: CCK-8 Assay, Inhibition, Cell Culture, Flow Cytometry, Expressing, Western Blot, Control

Journal: Molecular Medicine

Article Title: Celastrol promotes DNA damage and apoptosis in uterine corpus endometrial carcinoma via promotion of KAT2B-mediated RBPJ acetylation and repression of MCM4 transcription

doi: 10.1186/s10020-025-01082-z

Figure Lengend Snippet: Celastrol functions in UCEC through KAT2B. ( A ) After infection of lentivirus expressing shKAT2B or shNC, Ishikawa cells were treated for 24 h with 10 µM of Celastrol. Cell viability was detected by CCK8 kit. ( B ) Expression of CDK2, cyclin E and p21 in cells was determined. ( C ) Cell apoptosis was analyzed using TUNEL staining. ( D ) Representative images of γ-H2AX IF staining. All the values are mean ± SD. ∗∗ P < 0.01 vs. the control group. ## P < 0.01 vs. the Celastrol + shNC group

Article Snippet: The human endometrial cancer cell lines Ishikawa, HEC-1-B, KLE and HEC-1-A were obtained from iCell Bioscience Inc (Shanghai, China).

Techniques: Infection, Expressing, TUNEL Assay, Staining, Control